Genetic complementation problem

Genetic complementation problem

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I've attached the picture of the problem.

SO, based on the information I managed to assemble two large cistrons. (if the combination of two mutants yield (-) mutations are on the same chromosome)

A)so my cistrons are:



Note: somehow 4 and 7 is present in both cistrons

B) they ask us what mutation could cause two different cistrons have the same mutants

I was thinking maybe an insertion? i just dont get how it's even possible since if they have the same mutants doesnt it mean they overlap and thus are on the same chromosome??

I would appreciate if you could explain me what type mutation could cause it and check if I did the complementation correctly

  1. bacteriophage T4 has a single chromosome (approx. 200 kb in length). The chromosomes are terminally redundant, so the genetic map is circular, even though the physical map is linear (as I recall).
  2. A mutation in E. coli (or its viruses) that affects two adjacent protein-coding genes is said to be 'polar'. This indicates that the two genes in question form part of an operon. A strong loss-of-function mutation in the promoter for the operon will typically affect the expression of all the genes in the operon. In your example there are two cistrons, or genes, in the rII locus of T4.

It is possible that the data for this question are taken directly from the published literature. I suggest you search PubMed for papers by Seymour Benzer, from the 1950s.

Watch the video: Genetic Complementation (August 2022).